Recombinant human git1 protein
Webb5 maj 2000 · The GIT1 protein was identified in a two-hybrid screen with G protein-coupled receptor kinase 2 (GRK2) . The mRNA for the highly similar GIT2 protein, ... The human GIT2-short/6xHis ... ARF-GAP1, Δ45GIT1, Δ45ARF-GAP1, and ARF proteins. Recombinant GIT1/6xHis (lane 1), GIT2-short/6xHis (lane 2), ARF-GAP1/6xHis ... WebbHuman GIT1 Recombinant protein (His tag) Documents. DataSheet; Material Safety Data Sheets (MSDS) Source/Expression Host. E. coli. Expression Plasmid/cDNA. 471-640 aa encoded by BC067358 was inserted into PET30a vector. Protein Species. Human. Activity. Not tested. Validations. Recombinant human GIT1 SDS-PAGE.
Recombinant human git1 protein
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WebbARF GTPase-activating protein GIT1 is an enzyme encoded by the GIT1 gene in humans. It is a GTPase-activating protein (GAP) for the ADP ribosylation factor (ARF) family of … WebbHuman GIT1 Protein Lysate 20ug from Innovative Research is provided as a Lyophilized powder. This is a Recombinant Protein Lysate produced in HEK293T cells. This protein lysate can be reconsituted using SDS Sample Buffer. Once reconsituted, aliquot and store any remaining material at -20C to avoid repeated freeze-thaw
Webb1 apr. 2015 · Human c-Raf recombinant protein (rc-Raf) was purchased from GeneTex (Irvine, CA). ... To determine direct interactions between Ras and MAT2B or GIT1, human rRas protein immobilized to protein A/G plus agarose was incubated with rMAT2B or IVT GIT1 alone or together for 16 hours and beads were washed. Webb27 maj 2024 · GIT1 CKO mice display impaired intramembranous bone formation of defects on day 7 post-injury. (A) Representative images of micro-CT reconstruction of injured tibias (top panel) and mineralized callus (lower panel) in the defect area of indicated groups.(B–E) BV/TV (%) (B), Tb.N (C), Tb.Sp (D), and Tb.Th (E) of the mineralized bone …
WebbAll lanes : Anti-GIT1 antibody [EPR10368] at 1/1000 dilution Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate Lane 2 : G1T1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate Lane 3 : Human brain tissue nuclear fraction lysate Lane 4 : SH-SY5Y (Human … WebbUnlike most other signaling pathways, both the Notch ligands and the receptors are transmembrane protein 1; therefore, the Notch pathway is initiated by a receptor–ligand interaction between two neighboring cells. 24 It has also been reported that the Notch signaling pathway can also occur in the cytoplasmic membrane of the same cell. 25 In …
WebbObject Promotion of the repair and regeneration of damaged adult neurons is a major goal of neurological science. In this study, the effects of G protein–coupled receptor kinase interacting protein 1 (GIT1) overexpression in human neuron cells were tested in human neuronal cells by using an adenoviral vector. Methods A recombinant GIT1 and …
Webb1 apr. 2015 · PDF Methionine adenosyltransferase 2B (MAT2B) encodes for variant proteins V1 and V2 that interact with GIT1 to increase ERK activity and growth in ... Recombinant human MAT2B protein (rMAT2B) ... flights to haa alifu atollWebbG protein-coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved, ... Recombinant Human GIT2 Protein, Myc/DDK-tagged, C13 and N15-labeled: HEK293T: Myc/DDK: Inquiry: Mouse: Git2-3216M: Recombinant Mouse Git2 Protein, Myc/DDK-tagged: HEK293T: Myc/DDK: flights to haiti from fllWebbGIT1 contains an ARFGAP domain, Anykrin repeats, and a GRK-interacting domain. The Arf-GAP domain, which enables it to act as a GTPase activating protein (GAP) for the Arf … flights to hahn germanyWebbRecombinant proteins are widely used as reagents in laboratory experiments and to generate antibody probes for examining protein synthesis within cells and organisms. [4] Many additional practical applications of recombinant DNA are found in industry, food production, human and veterinary medicine, agriculture, and bioengineering. [4] cheryl eatonWebb30 mars 2007 · One role for GIT proteins is to link the PIX family of Rho guanine nucleotide exchange factors and their binding partners, the p21-activated protein kinases, to remodeling focal adhesions by interacting with the focal adhesion adaptor protein paxillin. We here identified the C-terminal domain of GIT1 responsible for paxillin binding. cheryl eatockWebbRecombinant Proteins; Protein Markers/ Ladders; Protease/Phosphatase Inhibitors; Secondary Antibodies; Isotype Controls; Conjugations; ... C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GIT1) polyclonal Antibody, Unconjugated (bs-55084R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37 ... flights to hailey idahoWebbGIT1 (ARF GTPase-activating protein GIT1) is a 95 kDa protein that belongs to ADP ribosylation factor family and is localized to focal adhesions, cytoplasmic complexes … cheryl eaton obituary