Npu trans-splicing split intein
Web24 feb. 2006 · Protein trans-splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme (Npu DnaE) was demonstrated here with non-native exteins in Escherichia coli.Npu DnaE possesses robust trans-splicing activity with an efficiency of >98%, which is superior to that of the DnaE intein from Synechocystis sp. strain … Web24 feb. 2006 · Npu DnaE possesses robust trans -splicing activity with an efficiency of >98%, which is superior to that of the DnaE intein from Synechocystis sp. strain PCC6803 ( Ssp DnaE). Both the N- and C-terminal parts of the split Npu DnaE intein can be substituted with the corresponding fragment of Ssp DnaE without loss of trans -splicing …
Npu trans-splicing split intein
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Web1 nov. 2011 · Split inteins carry out a naturally occurring process known as protein trans -splicing, where two protein fragments bind to form a catalytically competent enzyme, … Web24 feb. 2006 · Protein trans-splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme (Npu DnaE) was demonstrated here with non-native exteins in …
Web24 feb. 2016 · Protein trans-splicing (PTS) by split inteins has found widespread use in chemical biology and biotechnology. Herein, we describe the use of a consensus design … Web10 feb. 2009 · The most important properties of the split intein tools are (1) the yield of the protein trans-splicing reaction, (2) its rate constant, (3) the solubility of the intein fragments, and (4) their tolerance towards different fused “substrate” exteins.Currently, the probably most widely used split intein is also the Ssp DnaE intein. It has proven to …
Web20 mrt. 2006 · Protein trans-splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme (Npu DnaE) was demonstrated here with non-native exteins in … Webfirst-order protein trans-splicing rate for the Ssp DnaE intein (assuming a fast fragment association and a rate-determining slower trans-splicing reaction) was determined to be …
Web13 jan. 2009 · DnaE intein from Nostoc punctiforme (Npu) is one of naturally occurring split inteins, which has robust protein splicing activity.Highly efficient trans-splicing activity of NpuDnaE intein could widen various biotechnological applications.However, structural basis of the efficient protein splicing activity is poorly understood. As a first step toward better …
Web7 jul. 2009 · Inteins come in 2 flavors—cis splicing inteins are single polypeptides that are embedded in a host protein, whereas trans-splicing inteins (herein called split inteins) are separate polypeptides that mediate protein splicing after the intein pieces and their protein cargo associate (8, 9) (Fig. 1A).Despite the many applications of split inteins in chemical … intel iris xe max vs nvidia geforce mx450WebSplit inteins are expressed as two separate polypeptide fragments and trans-splice upon subunit association. Split inteins have found use in biotechnology applications but their … john angolia collection for auctionintel iris xe memory sizeWeb28 aug. 2015 · Protein splicing is mediated by inteins that auto-catalytically join two separated protein fragments with a peptide bond. Here we engineered a genetically encoded synthetic photoactivatable intein (named LOVInC), by using the light-sensitive LOV2 domain from Avena sativa as a switch to modulate the splicing activity of the split DnaE intein … intel® iris® xe max graphics驱动Web20 mrt. 2006 · DOI: 10.1016/j.febslet.2006.02.045 Corpus ID: 5199441; Highly efficient protein trans‐splicing by a naturally split DnaE intein from Nostoc punctiforme @article{Iwai2006HighlyEP, title={Highly efficient protein trans‐splicing by a naturally split DnaE intein from Nostoc punctiforme}, author={Hideo Iwai and Sara Z{\"u}ger and … john anglin wikipediaWebProtein trans-splicing (PTS) by split inteins has found widespread use in chemical biology and biotechnology. Herein, we describe the use of a consensus design approach to … intel iris xe microsoft flight simulatorWebThe most important properties of the split intein tools are (1) the yield of the protein trans-splicing reaction, (2) its rate constant, (3) the solubility of the intein fragments, and (4) their tolerance to-wards different fused ‘‘substrate” exteins. Currently, the probably most widely used split intein is also the Ssp DnaE intein. It ... john angotti attorney morgantown wv